Sample viability with Flow Cytometry is of the utmost importance. For example, if a shipment is delayed or if a specimen is held overnight, please do not put it in the refrigerator. Instead, add 1:1 RPMI fluid to the specimen and hold overnight at room temperature.
We find that under these conditions, the specimen stays viable for 4-5 days. We instruct our International Clients to do this protocol all the time. This allows the cells to get some glucose but does not shock them with a cold temperature and contributes to potentially erroneous results.
FFPE tissue (unstained slides are preferred)
Please include a pathologist-marked H&E slide, as well as the pathology report.
Note: If possible, the lesional area of interest should be under 22 x 22 mm in size and within 10 serial sections of the provided H&E slide to ensure detection of any abnormalities.
Paraffin embedded tissue should be fixed in 10% neutral buffered formalin fixed a minimum of 6 hours.
A minimum of 2 unstained slides at 4 microns thickness per FISH probe ordered.
Specimen slides should be stored and sent at ambient temperature using overnight shipping.
