FISH Probes / Panels by Disease Application
Update on Paraffin FISH Samples:
- Submit slide mounted paraffin sections only (marked H&E, 5 to 8 paraffin sections cut at 4 microns).
All Lymphoma probe panels are also available for paraffin-embedded specimens. For other FISH probe sets, please inquire with the laboratory.
Plasma cell enrichment performed on all MM/MGUS specimens.
FISH Turn Around Times:
Standard interphase 2 – 5 days
Paraffin embedded 5 – 7 days
For Bone Marrow Aspirate and Peripheral Blood specimens send 1-3ml of sample in a Na Heparin Green Top tube.
| FISH Panels | ||
|---|---|---|
| Panel | Probes | Validated for use in Paraffin |
| B-ALL, BCR-ABL1 -like(Ph-like) Panel | CRLF2, JAK2, IGH, EPOR | |
| B-ALL Panel (Adult) | BCR/ABL1, MYC/IGH, TEL/AML1(ETV6/RUNX1), MLL(KMT2A), ABL1, ABL2 | |
| B-ALL Panel (Pediatric) | BCR/ABL1, MYC/IGH, TEL/AML1(ETV6/RUNX1), MLL(KMT2A), ABL1, ABL2, Cen4/Cen10, PBX1/TCF3, PDGFRB, TP16(CDKN2A)/D9Z3 | |
| AML Panel (Pediatric) | AML1/ETO (RUNX1T1/RUNX1), PML/RARA, CBFB, MLL, Del(7q), EV1(MECOM), NUP98 (11p15), DEK/NUP214 | |
| AML Supplemental Panel | BCR/ABL1, EVI1, DEK/NUP214, NUP98 | |
| AML Panel (Adult) | AML1/ETO (RUNX1T1/RUNX1), PML/RARA, CBFB, MLL, Del(7q), Del(20q), + D8Z2 | |
| T-cell Lymphoma | ALK, TCRAD (for TRA & TRD), MYB/D6Z1, TCRB (for TRB) | |
| B-cell Lymphoma Panel (NHL Panel) | BCL6, MALT, BCL1 (CCND1), IGH/BCL2 |
x
|
| Burkitt’s Lymphoma Panel | MYC/IGH, MYC |
x
|
| Double/Triple Hit Lymphoma (DHL Panel) | MYC, MYC/IGH, IGH/BCL2, BCL6 |
x
|
| CML Panel | BCR/ABL1 | |
| CLL Panel with BCL1 (CCND1) | D13S319/13q34, D12Z3, ATM, TP53,MYB, CCND1/IGH | |
| CLL Panel without BCL1 (without CCND1) | D13S319/13q34, D12Z3, ATM,TP53 | |
| MDS Panel | Del(5q)(EGFR1), Del(7q), Del(20q)+D8Z2, TP53/D17Z1 | |
| MM/MGUS Panel | TP53/D17Z1, FGFR3/IGH, D13S319/13q34, CCND1/IGH, Cen3/Cen15, CDKN2C/CKS1B(1p/1q) | |
| MM Panel reflexive probes for IGH gain | IGH-breakapart, IGH/CCND1, IGH/MAF, IGH/CCND3, IGH/MAFB, IGH/MYC | |
| Eosinophilia/MPN FISH Panel | PDGFRA, FIP1L1, CHIC2, D8Z2, ASS1, PDGFRB, FGFR1, JAK2 FISH probe, BCR/ABL1 | |
| IRF4 Rearrangement for Large B-cell lymphoma or ALK-negative anaplastic large cell lymphomas | IRF4/DUSP22/D6Z1 | |
| Burkitt-like lymphoma with 11q aberration | CEP164/ETS1/FGF19(11q23.3/11q24.3/11q13.3) | |
| T-ALL Panel | BCR/ABL1, KMT2A (MLL), TCRB (for TRB), TCRAD (for TRA & TRD), TP16(CDKN2A)/D9Z3, MYC | |
| Pediatric-type Follicular (PTFL) | TNFRSF14, ABL2 | |